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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Fungal Genet Biol. 2013 Jul 16;0:10.1016/j.fgb.2013.07.002. doi: 10.1016/j.fgb.2013.07.002

Table 1.

Vectors, selective markers, and primers used in this study.

Vector Selective marker Primers
pDONR 221-AnargB argB A. nidulans ArgB1-ggggacaagtttgtacaaaaaagcaggctaagctttatttcgcggtttt
ArgB2-ggggaccactttgtacaagaaagctgggtgtcgacctacagccattggg
pDONR 221-AnpyrG pyrG A. nidulans attB1-AnpyrG5F-ggggacaagtttgtacaaaaaagcaggctgcctatgccagcattgagcaatg
attB2-AnpyrG3R-ggggaccactttgtacaagaaagctgggtcagtggagcgaaccaattctctc
pDONR 221-AfpyroA pyroA A. fumigatus attBpyroA1-ggggacaagtttgtacaaaaaagcaggctaagttgatcgatgaatgacattc
attBpyroA2-ggggaccactttgtacaagaaagctgggtctaaggcacccagcatgagtgc
pDONR 221-AfpyrG pyrG A. fumigatus attB1-AfpyrGF-ggggacaagtttgtacaaaaaagcaggctgcctcaaacaatgctcttcac
attB2-AfpyrGR-ggggaccactttgtacaagaaagctgggtattctgtctgagaggaggcac
pDONR 221-HPH HPH Escherichia coli attB1-HPH F-ggggacaagtttgtacaaaaaagcaggctgaattcgtcgacgttaactgg
attB2-HPH R-ggggaccactttgtacaagaaagctgggtgaattcgtcgacgttaactgc
pDONR 221-BAR BAR Streptomyces hygroscopicus attB BAR 1-ggggacaagtttgtacaaaaaagcaggctgcaagcccagatgagaaccgac
attB BAR 2-ggggaccactttgtacaagaaagctgggtcggagagagttgaacctggacg
pDONR A pyroA A. fumigatus pyroA F-GCGCGCACTAGTgcatcccaaggggaaggtagc
pyroA FR-ctagcgttaacgcgagagtagggaactgcctaaggcacccagcatgagtgc
pDONR221 FFA-cagttgacggcactcatgctgggtgccttaggcagttccctactctcgcgt
pDONR221 R1-GCGCGCACTAGTaattgcgttgcgctcactgcc
pDONR G pyrG A. fumigatus pyrG F-GCGCGCACTAGTatgctcttcaccctcttcgcg
pyrG FR-ctagcgttaacgcgagagtagggaactgccctgagaggaggcactgatgcg
pDONR221 FFG-ttggcatcacgcatcagtgcctcctctcagggcagttccctactctcgcgt
pDONR221 R1-GCGCGCACTAGTaattgcgttgcgctcactgcc
pDONR NG pyrG A. nidulans AnpyrG F-GCGCGCACTAGTctatgccagcattgagcaatg
AnpyrG FR-gagtagggaactgccagtggagcgaaccaattctct
pDONR221 FFG-AN-ttggttcgctccactggcagttccctactctcgcgt
pDONR221 R1-GCGCGCACTAGTaattgcgttgcgctcactgcc
pDONR B argB A. fumigatus AfargB F-GCGCGCACTAGTaacgctgacgatgaggctgat
AfargB FR-gagtagggaactgccaaacggttcttggctggaagg
pDONR221 FF-AfargB-agccaagaaccgtttggcagttccctactctcgcgt
pDONR221 R1-GCGCGCACTAGTaattgcgttgcgctcactgcc
pDONR HPH A pDONR HPH B HPH Escherichia coli HPH F-ACTAGTgaattcgtcgacgttaactgc
HPH FR-ctagcgttaacgcgagagtagggaactgcccgtcgacgttaactggttccc
pDONR BAR BAR (Bernhard Straubinger 1992) Streptomyces hygroscopicus BAR F-GCGCGCACTAGTaagcccagatgagaaccgac
BAR FR-gagtagggaactgccggagagagttgaacctggacg
pDONR221 FF-BAR-ggttcaactctctccggcagttccctactctcgcgt
pDONR221 R1-GCGCGCACTAGTaattgcgttgcgctcactgcc
pDONR AMA pyr4 Neurospora crassa ccd F-GGGGCATGCcccaaataatgattttatttt
ccd R-GGGGGTACCaaataatgattttattttgactga
pDONR AMA/HPH HPH Escherichia coli pDONR HindIII F-GCGCGCAAGCTTaagcggaagagcgcccaatac
pDONR 221 R3-GCGCGCAAGCTTggcggtaatacggttatccacagaatcag
pDONR P4-P1R-mkkA 5' FR mpkkA 1-ggggacaactttgtatagaaaagttgaaGCGGCCGCtcaatatatcgtcaactcaga
mpkkA 2- ggggactgcttttttgtacaaacttgcggcgagactgttttgcctctc
pDONR P2R-P3-mkkA 3' FR mpkkA 3- ggggacagctttcttgtacaaagtggaactctttggtctttgacgctta
mpkkA 4- ggggacaactttgtataataaagttgcacttcccggggtctacttcgt
pDONR A-mkkA CR mkkA C1 - ggggacaagtttgtacaaaaaagcaggctaagcggccgcgagcccagaccctagatatcg
mkkA C2- ggggaccactttgtacaagaaagctgggtctacagcctgcaagcttttcag
pDONR AMA-gliZ gliZ attB 1- ggggacaagtttgtacaaaaaagcaggctgcgaccgcagctgattggag
gliZ attB 2- ggggaccactttgtacaagaaagctgggtcgattccctttgtgccgcc

attB sites are underlined. All primers are listed 5' to 3'. Bolded sequences are extension regions for f-PCR. Uppercase regions are SpeI site in all primers, except ccd F (SphI), ccd R (KpnI), pDONR HindIII F (HindIII), pDONR R3 (HindIII), and mpkkA 1 (NotI). All nutritional markers are flanked by their respective promoter and terminator regions. HPH is preceded by the A. nidulans trpC promoter region (http://www.fgsc.net/fgn41/carroll.html), and BAR is flanked by the amdS promoter region from A. nidulans (with increased expression from I9 and I66 mutations) and the glucoamylase terminator from A. niger (Bernhard Straubinger 1992).