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. Author manuscript; available in PMC: 2014 Oct 4.
Published in final edited form as: J Proteome Res. 2013 Sep 19;12(10):10.1021/pr400307u. doi: 10.1021/pr400307u

Figure 4.

Figure 4

iTRAQ protein quantitation consensus among four mass spectrometers, relationship of data tightness with decreasing Mascot Protein Score. iTRAQ fold-change ratios scatter increases with decreasing Mascot Protein Score. Red, TripleTOF #1; Blue, TripleTOF #2; Green, LTQ- Orbitrap Velos; Purple, Q Exactive. X axis represent the Mascot Protein Score from TripleTOF #2 for each protein plotted. X axis of panel A is in log. X axis of B and C are linear. Proteins with Mascot Protein Scores >100 and the absolute ratios of cases to control of |log2|>0.35 in fractions 6, 18 and 24 of experiment #2 are plotted.