Figure 6.

S5-DBD-PA suppresses the viability and the growth of tumor cells. (a) A431 epidermal, PC-3 prostate and HCT116 colorectal carcinoma cell lines were infected with SiEW-lentiviral vectors encoding either S5-DBD-PA, hTRX or the empty vector. Over a period of 20 days cell viability and concomitant growth was monitored by XTT assay. Results are shown as the percentage of viable cells compared to the mock-treated control. Stat5 and transgene expression was verified by western blot 7 days after infection using antibodies recognizing either total or tyrosine phosphorylated Stat5 protein and a flag tag antibody. (b) The same experiments were done with unstimulatedT-47D breast cancer cells. Protein expression was analyzed after 7 days with and without 30 min Prl stimulation. (n = 3; Ø ± SD). Significantly reduced XTT-values in comparison to empty vector expressing cells are indicated. * p < 0.05, ** p < 0.01, *** p < 0.001 (2-way-ANOVA with Bonferroni correction).