Figure 7.

Suppression of tumor cell growth and viability by transduction of recombinant S5-DBD-PA. S5-DBD-PA was transduced into the four tumor celllines A431, PC-3, T-47D and HCT116 by adding 0.5, 1, 1.5 µM of recombinant S5-DBD-PA to the culture medium. The treatment with the scaffold control protein (hTRX) in a 1.5 µM concentration as well as with the same volume of protein-solvent (dialysis buffer) and PBS served as negative controls. Medium and peptides were replaced daily and cell viability and concomitant growth was determined by XTT assay. Results are shown as the percentage of viable cells compared to the PBS control. (n = 3; Ø ± SD) Significantly reduced XTT-values in comparison to protein-solvent treated cells are indicated. * p < 0.05, ** p < 0.01, *** p < 0.001 (2-way-ANOVA with Bonferroni correction).