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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: Circ Res. 2013 May 29;113(3):10.1161/CIRCRESAHA.113.301552. doi: 10.1161/CIRCRESAHA.113.301552

Figure 4. ABCB10 promotes mitochondrial ALA export.

Figure 4

A,B, Cellular (A) and mitochondrial (B) heme levels in ABCB10 knockdown cells incubated with 1.2mM ALA for 6 hours (n=6-18). ALA treatment reversed the decrease in heme levels associated with ABCB10 knockdown (as shown in Fig. 1A and B). C-E, Activities of heme-containing enzymes in cells with ABCB10 knockdown following supplementation with ALA (n=4-6). ALA supplementation restored the activities of heme-containing enzymes to control levels in ABCB10 knockdown cells. F, Cellular heme levels with ABCB10 knockdown and supplementation with 500μM methyl-succinate (m-succinate) or vehicle (n=6). G, Cellular heme levels with overexpression of zALAS2 and knockdown of ABCB10 (n=6). Data are presented as mean ± SEM. * p<0.05 vs. control, # p<0.05 vs. zALAS2 overexpressing, control shRNA transfected cells.