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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: Circ Res. 2013 May 29;113(3):10.1161/CIRCRESAHA.113.301552. doi: 10.1161/CIRCRESAHA.113.301552

Figure 5. Hypoxic upregulation of ABCB10 is required for cell survival.

Figure 5

A, Western blot analysis showing that ABCB10 is upregulated by hypoxia in H9c2 cells, and this increase is prevented by treatment of hypoxic cells with ABCB10 siRNA (n=3). B, Heme levels in H9c2 subjected to 48 hours of hypoxia (1% O2) or normoxia, with or without suppression of hypoxic ABCB10 upregulation by siRNA (n=6). C, MitoSox staining (red) of H9c2 cells with and without reversal of ABCB10 upregulation by siRNA subjected to 48 hours of hypoxia followed by 30 minutes of reoxygenation. Nuclei are counterstained with Hoescht (blue). Representative images are shown on the left, and quantification is presented on the right (n=6, 4 fields per sample). D,E, Cell death as assessed by propidium iodine (PI) and Annexin V staining and quantified by flow cytometry in H9c2 cells with and without ABCB10 siRNA treatment subjected to hypoxia-reoxygenation injury as in C (n=9) (D), or with additional incubation with 200μM hydrogen peroxide for 12 hours in hypoxia prior to reoxygenation (n=6) (E). Data are presented as mean ± SEM. * p<0.05 vs. control.