Figure 1. EM reconstruction of Tetrahymena telomerase holoenzyme and subunit localization.
a, Holoenzyme subunits and domains (top) and TER secondary structure (below). b, Representative class averages of negative staining EM and cryoEM images of TERT-f telomerase. c, 3D reconstruction of Teb1-f telomerase (front and side views) and class averages of affinity labeled telomerase particles. Lines with circle heads indicate attachment point of Fab (red arrows) and MS2cp (white arrow). Side-lengths of class averages in this and subsequent figures are 350 Å. d, Subunit schematic (front view).