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Multiplex PCR of P. aeruginosa isolates. Lane M: 100 bp DNA ladder (Fermentas Cat# SM323). Lane 1-3: Multiplex PCR of P. aeruginosa showing amplicons of 16S rDNA (618 bp), oprL (504), ETA (397bp), ivnA internal control (284bp) and gyrB gene fragments (222 bp). Lane 4 and 5: Multiplex PCR of negative control (Proteus mirabilis) showing amplified product of internal control ivnA gene fragment (284bp) only. Lane 6: Negative control (without template DNA).
