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. 2013 Oct 15;126(20):4572–4588. doi: 10.1242/jcs.118232

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — Analysis of focal adhesion parameters in Rac1^−/− MEFs. (A–F) Assessment of focal adhesion parameters from vinculin stainings. Representative images of Rac1^fl/fl (A,A′) and Rac1^−/− (B,B′) cells stained with vinculin antibodies (A,B). Images were thresholded as detailed in Materials and Methods; a binarized threshold (A′,B′) is shown to represent data extraction. From these images, number of adhesions per cell (C), number of adhesions per cell area (D), focal adhesion intensity (E) and focal adhesion size (F) were calculated. 30 cells were analyzed for each cell type.