Figure 1.

Imaging the AL. a) Anatomical model of the honeybee AL (generated from the average-shape atlas [31]), illustrating the view on the AL in calcium imaging experiments. Landmark glomeruli are labelled according to [30]. b) Schematic for a calcium imaging movie. The image on top of the stack is the ratio of images recorded with excitation light at wavelengths 340 nm and 380 nm, which is the input signal for the algorithm. For orientation, positions of the landmark glomeruli are marked by glomerulus numbers/labels. Constructing glomerular maps, as with the algorithm described in this work, facilitates identification of the glomeruli.