Table 1.
Minimum inhibitory concentration (MIC) of the extract, fractions and compounds (1–8) from E. robustum against fungi (C.a, C.A, A.f, C.n) and bacteria (S.a, P.a, E.f, E.c)
| Samples |
Fungi (MIC values) |
Bacteria (MIC values) |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
C.a |
C.A |
A.f |
C.n
a
|
Average |
S.a |
P.a |
E.f |
E.c |
Average |
|||||
| 16 h | 24 h | 16 h | 24 h | 16 h | 24 h | 24 h | 36 h | |||||||
|
Extract and Fractions (mg/ml) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Crude extract |
1.25 |
1.25 |
1.25 |
1.25 |
0.16 |
0.63 |
0.63 |
0.63 |
0.88 |
0.08 |
0.31 |
0.08 |
0.08 |
0.14 |
| Fraction A |
0.63 |
0.63 |
0.16 |
0.32 |
0.32 |
0.63 |
1.25 |
1.25 |
0.65 |
0.02 |
0.31 |
0.04 |
0.04 |
0.10 |
| Fraction B |
1.25 |
1.25 |
0.32 |
0.63 |
0.08 |
0.63 |
0.08 |
0.63 |
0.61 |
0.16 |
0.31 |
0.08 |
0.08 |
0.16 |
|
Compounds (μg/ml) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Robusflavone A (1) |
250 |
250 |
65 |
65 |
65 |
125 |
250 |
250 |
165 |
250 |
63 |
250 |
250 |
203.3 |
| Robusflavone B (2) |
125 |
125 |
65 |
65 |
65 |
125 |
65 |
65 |
88 |
125 |
63 |
63 |
125 |
94 |
| Orostachyscerebroside A (3) |
250 |
250 |
130 |
250 |
130 |
250 |
250 |
250 |
220 |
125 |
63 |
125 |
125 |
109.5 |
| Stigmasterol (4) |
125 |
125 |
125 |
125 |
65 |
65 |
125 |
125 |
110 |
125 |
63 |
250 |
250 |
172 |
| 1-O-Heptatriacontanoyl glycerol (5) |
250 |
250 |
65 |
65 |
125 |
125 |
250 |
250 |
173 |
125 |
63 |
125 |
125 |
109.5 |
| Eicosanoic acid (6) |
250 |
250 |
125 |
125 |
125 |
125 |
> 250 |
> 250 |
188 |
63 |
63 |
125 |
125 |
94 |
| 3-O-β-D-Glucopyranoside of sitosterol (7) |
125 |
125 |
125 |
125 |
65 |
125 |
65 |
125 |
110 |
125 |
63 |
63 |
125 |
94 |
| 6-Prenylpinocembrin (8) |
125 |
125 |
65 |
65 |
125 |
250 |
> 250 |
> 250 |
157 |
31.3 |
63 |
63 |
63 |
55.1 |
|
Controls (μg/ml) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Amp B |
30 |
30 |
10 |
30 |
65 |
125 |
> 250 |
> 250 |
99 |
- |
- |
- |
- |
- |
| Gen |
- |
- |
- |
- |
- |
- |
- |
- |
- |
< 3.91 |
< 3.91 |
< 3.91 |
< 3.91 |
< 3.91 |
| DMSOb | na | na | na | na | na | na | na | na | na | na | na | na | ||
C.a: Candida albicans (isolate), C.A: Candida albicans (ATCC), C.n: Cryptococcus neoformans, A.f: Aspergillus fumigatus, E.c: Escherichia coli, E.f: Enterococcus faecalis, S.a: Staphylococcus aureus, P.a: Pseudomonas aeruginosa, Amp B: Amphotericin B, Gen: Gentamicin, aWith this microorganism little reaction was observed after 16 h and values were measured after 24 and 36 h, bThe final concentration of DMSO used was less than 10%, na: not active.
The results are the means of three replicates for fungi and two replicates for bacteria and the standard deviation was zero.