Table 2.
Total activity in ml/g of crude extract and fractions (A and B) from E. robustum calculated by dividing mass in g of extract or fractions with their MIC values[20]
| Samples |
Fungi |
Bacteria |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
C.a |
C.A |
A.f |
C.n |
Average |
S.a |
P.a |
E.f |
E.c |
Average |
|||||
| 16 h | 24 h | 16 h | 24 h | 16 h | 24 h | 24 h | 36 h | |||||||
|
Extract and Fractions |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Crude extract |
4000 |
4000 |
4000 |
4000 |
31250 |
7937 |
7937 |
7937 |
8883 |
62500 |
16129 |
62500 |
62500 |
50907 |
| Fraction A |
1429 |
1429 |
5625 |
2813 |
2813 |
1429 |
720 |
720 |
2123 |
45000 |
2903 |
22500 |
22500 |
23226 |
| Fraction B |
560 |
560 |
2188 |
1111 |
8750 |
1111 |
8750 |
1111 |
3018 |
4375 |
2258 |
8750 |
8750 |
6033 |
|
Compounds |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Robusflavone A (1) |
4 |
4 |
13.4 |
13.4 |
13.4 |
8 |
4 |
4 |
8 |
4 |
16 |
4 |
4 |
7 |
| Robusflavone B (2) |
8 |
8 |
13.4 |
13.4 |
13.4 |
8 |
13.4 |
13.4 |
11 |
8 |
16 |
16 |
8 |
12 |
| Orostachyscerebroside A (3) |
4 |
4 |
7.7 |
4 |
7.7 |
4 |
4 |
4 |
4.5 |
8 |
16 |
8 |
8 |
9.1 |
| Stigmasterol (4) |
8 |
8 |
8 |
8 |
13.4 |
13.4 |
8 |
8 |
9 |
8 |
16 |
4 |
4 |
8 |
| 1-O-Heptatriacontanoyl glycerol (5) |
4 |
4 |
13.4 |
13.4 |
8 |
8 |
4 |
4 |
7 |
8 |
16 |
8 |
8 |
10 |
| Eicosanoic acid (6) |
4 |
4 |
8 |
8 |
8 |
8 |
< 8 |
< 8 |
7 |
16 |
16 |
8 |
8 |
12 |
| 3-O-β-D-Glucopyranoside of sitosterol (7) |
8 |
8 |
8 |
8 |
13.4 |
8 |
13.4 |
8 |
9 |
8 |
16 |
16 |
8 |
12 |
| 6-Prenylpinocembrin (8) | 8 | 8 | 13.4 | 13.4 | 8 | 4 | < 4 | < 4 | 8 | 32 | 16 | 16 | 16 | 20 |
C.a: Candida albicans (isolate), C.A: Candida albicans (ATCC), C.n: Cryptococcus neoformans, A.f: Aspergillus fumigatus, E.c: Escherichia coli, E.f: Enterococcus faecalis, S.a: Staphylococcus aureus, P.a: Pseudomonas aeruginosa.
The inverse of MIC in ml/mg was calculated for compounds (1–8).