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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Jul 11;33(9):10.1161/ATVBAHA.113.301894. doi: 10.1161/ATVBAHA.113.301894

Figure 5.

Figure 5

p47phox phosphorylation and the PX domain regulate CyPA and p47phox interaction. A and B, The interactions between CyPA and p47phox or actin were measured by immunoprecipitation and Western analysis in RASMC pretreated with Rottlerin (10 μmol/L) for 2 hours followed by AngII (10−7 mol/L) for 10 minute. C and D, Full length and PX domain deleted-mutant p47phox (p47phoxΔ) lentiviral particles are transduced into RASMC, and immunoprecipitation and Western analysis were performed to detect the interaction between CyPA and p47phox or actin. All results are from three independent experiments and data are mean±SEM. *P<0.05 vs 0 minute; #P<0.05 vs AngII.