Fig. 5.
Hh produced by multiple sources is required for follicle formation. (A-J) Ovarioles in which HhRNAi is driven by Bab1-Gal4 (A,B), 13C06 (C,D), 109-30 (E,F), Tj-Gal4 (G,H) or both Bab1-Gal4 and Tj-Gal4 (I,J) at 7 (A,C,E,G,I) or 21 (B,D,F,H,J) days after flies were shifted to 29°C to repress tub-Gal80ts and promote Gal4 activity. Tissue is stained for Fas3 (red) and with DAPI (blue). Brackets indicate the extent of the germarium. Arrows indicate follicle cell defects. (K) Quantification of follicle formation defects in ovarioles of each genotype. Each data point is the mean of at least three replicates. Ovarioles from 13C06, tub-Gal80ts; TM2/+ flies were used as the control. *P<0.05 compared with the control; error bars represent s.d. Total n values are greater than 200 ovarioles for each data point. Anterior is to the left. DATS, days after temperature shift. Scale bar: 5 μm.