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. 2013 Nov 15;140(22):4490–4498. doi: 10.1242/dev.098558

Fig. 6.

Fig. 6.

Multiple cell types have active Hh signaling. (A-F) Ovarioles from flies at 7 DATS that contain Ptc-pelican-GFP(nls), UAS-HhRNAi, tub-Gal80ts, and either no Gal4 driver (‘wild type’, A), Bab1-Gal4 (B), 13C06 (C), 109-30 (D), Tj-Gal4 (E) or Bab1-Gal4 and Tj-Gal4 (F) stained for GFP (green) to visualize Ptc-pelican-GFP(nls) expression and with DAPI (blue). (A-F′) GFP channel only. In wild type, GFP expression is bright in escort cells and tapers off toward the posterior in follicle cells. Germ cells are GFP-. In C, the germarium also includes UAS::CD8-GFP, which labels the membranes of cells expressing Gal4. Note the absence of the nuclear Ptc-GFP signal in these cells. White lines indicate the approximate range of Gal4 expression. Anterior is to the left. Scale bar: 5 μm.