Fig. 4.

Forelimb zeugopod muscles are disrupted in Hoxa11/d11 double mutants whereas stylopod muscles are unaffected. (A-D) Transverse section through the stylopod of E14.5 control (A) and Hox11 double-mutant forelimbs (C) stained for differentiated muscle (My32 antibody) shows no difference in muscle pattern in this region in the absence of Hox11 genes. The zeugopod of the Hox11 mutant (D) displays severe patterning defects compared with control (B). (E-H) 3D reconstruction of serial sections stained with My32 antibody using Amira software. Numbers denote specific muscles listed in the table below. Many dorsal muscle groups are merged (10/11, 12/13/14) or absent (15) and several ventral muscle groups are absent (17, 19, 21). h, humerus; r, radius; u, ulna.