. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Psychopharmacology (Berl). 2013 Jun 19;230(3):10.1007/s00213-013-3166-x. doi: 10.1007/s00213-013-3166-x

Table 1.

Alcohol’s effects on μ-opioid receptor binding and coupling in wt and Arrb2 knockout mice

		[³H]DAMGO binding		[³⁵S]-GTPgS accumulation
		F-value [DF]	p-value	F-value [DF]	p-value
mPFC
	G	1.87 [1,10]	n.s.	0.42 [1,8]	n.s.
	T	3.16 [1,10]	n.s.	0.98 [1,8]	n.s.
	G × T	4.80 [1,10]	n.s.	0.42 [1,8]	n.s.
AcbS
	G	0.12 [1,11]	n.s.	15.47 [1,13]	0.0012^**
	T	0.33 [1,11]	n.s.	7.02 [1,13]	0.0175
	G × T	0.99 [1,11]	n.s.	11.21 [1,13]	0.0041^*
AcbC
	G	0.06 [1,11]	n.s.	8.89 [1,16]	0.0106^*
	T	0.70 [1,11]	n.s.	1.29 [1,16]	n.s.
	G × T	0.49 [1,11]	n.s.	0.76 [1,16]	n.s.
CPu
	G	5.20 [1,14]	0.0387	20.70 [1,12]	0.0007^**
	T	1.28 [1,14]	0.2984	14.58 [1,12]	0.0024^*
	G × T	5.76 [1,14]	0.0309	13.29 [1,12]	0.0034^*
BNST
	G	0.97 [1,10]	n.s.	2.38 [1,12]	n.s.
	T	4.92 [1,10]	n.s.	4.01 [1,12]	n.s.
	G × T	2.26 [1,10]	n.s.	1.83 [1,12]	n.s.
CeA
	G	11.04 [1,15]	0.0046^*	11.67 [1,14]	0.0042^*
	T	17.51 [1,15]	0.0008^**	0.80 [1,14]	n.s.
	G × T	15.24 [1,15]	0.0014^*	11.47 [1,14]	0.0043^*
MeA
	G	10.19 [1,15]	0.0061^*	117.00 [1,14]	0.0001^***
	T	8.99 [1,15]	0.0090	20.75 [1,14]	0.0004^**
	G × T	0.01 [1,15]	n.s.	36.92 [1,14]	0.001^***
BLA
	G	10.30 [1,15]	0.0059^*	14.14 [1,11]	0.0032^*
	T	0.00 [1,15]	n.s.	1.32 [1,11]	n.s.
	G × T	0.84 [1,15]	n.s.	0.22 [1,11]	n.s.
BMA
	G	20.84 [1,15]	0.0004^**	1.20 [1,12]	n.s.
	T	10.11 [1,15]	0.0063	5.52 [1,12]	0.0368
	G × T	6.89 [1,15]	0.0192	11.11 [1,12]	0.0060^*
VTA
	G	0.75 [1,12]	n.s.	n.d.	n.d.
	T	0.02 [1,12]	n.s.
	G × T	0.45 [1,12]	n.s.

Statistical analysis was performed by 2-way ANOVA for the effects of genotype (G), alcohol treatment (T) and their interactions (G × T) in the respective brain regions. F-values with degrees of freedom [DF] and raw p-values are shown. In order to correct for multiple tests with family-wise error rate of 0.05, Holm’s corrected Bonferoni’s test was used. Corrected p-values are indicated: p < 0.05

^**

p < 0.001

^***

p < 0.0001

n.d. not detected. Brain regions: mPFC – medial prefrontal cortex, AcbC – nucleus accumbens core regions, AcbS – nucleus accumbens nucleus, shell region, BNST – bed nucleus of the stria terminalis, CPu – caudate putamen, CeA, MeA, BLA, BMA – central, medial, basolateral and basomedial nucleus of the amygdala, respectively, VTA- ventral tegmental area.