Figure 1.

Fibroblasts from BD patients have an attenuated response to Li. Vehicle, Li 1 mM or Li 10 mM was included in the growth medium for 48 h prior to, and continuously during 5 days of recording. Rhythmic Per2::luc expression was measured as counts per second (cps) with baseline subtraction. (a) Amplitude increased in cells from control (N=19, P<0.01), but not BD (N=19, P=0.32) subjects after Li 1 mM (error bars indicate 95% confidence intervals). (b) There was a non-significant trend toward greater period lengthening in controls compared with BD cells after Li 10 mM (error bars indicate 95% confidence intervals). Representative vehicle-treated (black) and Li-treated (red) rhythm traces are shown for representative control and BD cell lines at 1 mM (c, d) and 10 mM (e, f).