Figure 4.

Li improves rhythm resynchronization. Cellular rhythms (N=10 each BD and control) were measured without treatment until damped (dashed line, day 1–5 in panel a). In order to restore rhythms, medium change with Li 10 mM (red) or vehicle (black) was performed on day 5.5 (arrow in panel a). Omitting the signal artifact associated with media change, rhythms were then measured another 2 days (solid line in panel a). After media change, Li-treated cells had (b) significantly higher rhythm amplitudes (P<0.05), and (c) lower periods (P<0.001) compared with vehicle-treated cells. Following media change, period in Li-treated cells was in the circadian range (23.1±0.5 h (Ave±s.e.m.)); whereas period in the vehicle-treated cells was not (31.1±1.9 h (Ave±s.e.m.)).