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. 2013 Oct 17;69(Pt 11):1216–1219. doi: 10.1107/S1744309113025803

Figure 1.

Figure 1

Purification of S. aureus homoserine dehydrogenase. (a) A 12% SDS–PAGE profile of purified SaHSD. Lane M contains molecular-mass marker (labelled in kDa; Thermo Scientific), while lane P corresponds to freshly purified SaHSD. (b) Mass spectrum of purified SaHSD. Of the two peaks in this spectrum obtained on a MALDI–TOF/TOF mass spectrometer (Bruker Daltonics), the mass of the smaller peak is consistent with a dimeric species. The calculated molecular weight of the SaHSD monomer is 51 203 Da.