| 8.5 |
0.2 M magnesium acetate, 14%(w/v) PEG 8000, 0.2 M Bicine, 5% glycerol (200 µl of a 1:1 ratio of paraffin and silicone oil was overlaid on the mother liquor in the reservoir well) |
15% DMSO |
Hanging-drop vapour diffusion |
| 7.5 |
0.2 M magnesium acetate, 16%(w/v) PEG 8000, 0.1 M Tris–HCl, 3% glycerol (crystal soaked with 5 mM lysine and 4 mM NADP for 2 min) |
15% DMSO |
Hanging-drop vapour diffusion |
| 7.0 |
0.2 M magnesium acetate, 16%(w/v) PEG 3350, 0.1 M HEPES, 20% 2-propanol, 5% glycerol (crystal soaked with 5 mM serine for 2 min) |
15% DMSO |
Sitting-drop vapour diffusion |
| 6.5 |
0.2 M magnesium acetate, 16%(w/v) PEG 3350, 0.1 M sodium cacodylate, 5% glycerol (crystal soaked with 5 mM serine and 2 mM NADP for 2 min) |
15% DMSO |
Sitting-drop vapour diffusion |
| 6.0 |
0.2 M magnesium acetate, 22%(w/v) PEG 8000, 0.1 M bis-tris, 1 mM serine, 5% glycerol |
15% DMSO |
Microbatch |
