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. 2013 Sep 10;14(11):984–991. doi: 10.1038/embor.2013.142

Figure 5.

Figure 5

Sister chromatid cohesion constrains Rad52-YFP foci and undamaged chromatin. (A) Western blot showing efficient cleavage of Scc1::TEV on TEV induction in GA7757. (B,C) MSD analyses of spontaneous (B) and Zeocin-induced (C; 50 μg/ml for 1 h) Rad52-YFP foci after cleavage of cohesin (GA7757; 11 and 12 movies, respectively) compared with wild type (GA5820; 21 and 28 movies as in Fig 1). (D) MSD analysis of spontaneous Rad52-YFP foci in cells containing the Eco1-aid allele (GA8193) treated with DMSO (12 movies) or auxin (IAA; 14 movies). Only S-phase cells were analyzed. (E,F) MSD analysis of spontaneous and Zeocin-induced Rad52-YFP foci in tof1Δ cells (GA8160; 11 and 12 movies, respectively) compared with wild-type cells (GA5820; 21 and 28 movies, respectively). (G,H) MSD curves of LacO-tagged PES4 in GA1461 (wild type) and after cleavage of Scc1 (GA8112) in G1 (D; 26 and 22 movies, respectively) and S phase (E; 14 and 19 movies, respectively). The error bars on the MSD curves represent the s.e. MSD, mean square displacement; YFP, yellow fluorescent protein.