Figure 3.
Quantitative tPTAA spectral emission and Aβ40/42 ratio of induced Aβ deposits. The analysis is from the same mice as in Fig 2. For the APP23 host, all mice (n=5/extract) were included in the analysis; for the APPPS1 host, the two extremes were selected for analysis, that is, the 1.5-month-old mice that were analysed 1.5 months later, and the 3-month-old mice that were analysed 3 months later (total n=4/extract). (A) Quantitative emission spectra of the ratio of the emitted light at 534 nm and 631 nm. For methodological details see Fig 1. Mean and s.e.m. are indicated. ANOVA (host × extract) revealed significant main effects (host F(1,14)=12.0; P<0.01; Extract F(1,14)=67.1; P<0.001) and interaction (F(1,14)=6.8; P<0.05). Post hoc analysis revealed significant differences between APP23- and APPPS1-induced Aβ deposits in the APP23 host (P<0.001) and APPPS1 host (P<0.01). (B) Densitometric analysis of the Aβ40/Aβ42 ratio plotted for each laser-dissected tissue piece. For methodological details see Fig 1. Mean and s.e.m. are indicated. The dotted line indicates a ratio of 1. ANOVA (host × extract) revealed significant main effects (Host F(1.14)=175.9; P<0.001; extract F(1.14)=40.5; P<0.001) and interaction (F(1.14)=34.41; P<0.001). Post hoc analysis revealed a difference between the APP23- and APPPS1-induced Aβ deposits in the APP23 host (P<0.001) but failed to reach significance in the APPPS1 host (P>0.05). Aβ, β-amyloid peptide; ANOVA, analysis of variance; tPTAA, trimeric polythiophene acetic acid.
