FIGURE 4.
Binding of CRP by live virulent S. pneumoniae strain WU2. A, Binding in Todd-Hewitt broth. A fixed number of live bacteria were grown in the presence of increasing amount of CRP, as shown on the x-axis. Then, CRP that bound to bacteria was eluted and measured by ELISA. Amount of bound CRP is shown on the y-axis. B, Binding in mouse serum. A fixed number of live bacteria were grown in the presence of 2000 ng of WT or mutant CRP. Then, CRP that bound to bacteria was eluted and measured by ELISA. The amount of bound CRP is shown on the y-axis.