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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 May 23;33(8):10.1161/ATVBAHA.112.300985. doi: 10.1161/ATVBAHA.112.300985

Figure 4.

Figure 4

Negative modulation of Akt signaling accounts for antiproliferative action of TLM. Immunoblot analyses (A) and averaged densitometric data (B) show the effects of 100 μmol/L TLM on the dynamic phosphorylation of Akt (i and ii), MDM2 (iii), GSK3β (iv) and on CyclinD1 levels (v) over a time course of 72 hours in HUVEC. C, Representative immunofluorescence images (i), immunoblot analyses (iii) and respective averaged fluorescence intensity quantification (ii) and averaged densitometric data (iv) of nuclear p53 in HUVEC treated as in A. Scale bar: 50μm. n=5; for all data in B and C, P<0.05 vs DMSO by 2-way ANOVA for both the effect of time and treatment on fold-change.