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. Author manuscript; available in PMC: 2013 Nov 5.
Published in final edited form as: Methods Mol Biol. 2012;917:10.1007/978-1-61779-992-1_11. doi: 10.1007/978-1-61779-992-1_11

Fig. 3.

Fig. 3

We use an oil-filled injection system for the nuclear transplantations. The syringe and tubing are filled with mineral oil and the infusion pump depresses the syringe plunger, resulting in a constant, desirable flow rate (0.6 μL/min for X. laevis; 0.2 μL/min for X. tropicalis).