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. Author manuscript; available in PMC: 2013 Nov 5.
Published in final edited form as: Mol Carcinog. 2010 May;49(5):10.1002/mc.20613. doi: 10.1002/mc.20613

Figure 1. AMPK signaling correlates with p27 expression.

Figure 1

A, Lysates from Tsc2-null ELT-3 cells that were treated overnight with Compound C were immunoblotted using anti-p27, anti-phospho-AMPK-α (T172) and anti-AMPK-α antibodies. B, Lysates from NIH3T3 cells grown overnight in the absence of serum and glucose, treated with Compound C for 3h were immunoblotted using anti-p27 and anti-GAPDH antibodies. C, Protein lysates from neonatal and adult kidney and five independent kidney tumors from Eker rats (Tsc2Ek/+) were immunoblotted using anti-p27, anti-phospho-AMPK-α (T172), anti-AMPK-α, anti-AMPK-α1, anti-AMPK-α2, anti-phospho-S6 and anti-S6 antibodies.