Fig. 2.
Endocytosis of FM dye by AP180- and CALM KD-neurons. Live neurons expressing Syp::EGFP (green) and AP180shRNA (a; AP180 KD) or CALMshRNA (b; CALM KD) were incubated with fixable FM 4–64 (magenta) and then fixed. Although the Syp::EGFP puncta are considerably smaller in AP180- and CALM KD-neurons, these smaller puncta internalize the FM dye (white arrowheads). The experiment was repeated three times (Color figure online)