Table 1.
Yield of Products from LEE Induced DNA Damage.
| Samplea | Damageb | Bromine release | Base release | Phosphodiester bond cleavage | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
||||||||||||||
| TXT | TUT | %c | T | X(U) | Sum | %d | pT | Tp | pXT(pUT) | TXp(TUp) | Sum | %e | ratiof | |
| X=5BrU | 138 | 53.5 | 38.8 | 25.8 | 1.8(4.4) | 32.0 | 23.2 | 4.3 | 2.5 | 3.0(4.5) | 2.1(1.3) | 17.7 | 12.8 | 1.81 |
| X=T | 92 | 17.0 | 17.0 | 18.5 | 1.6 | 1.2 | 4.3 | 2.7 | 9.8 | 10.7 | 1.73 | |||
| X=U | 82 | 14.8 | 2.5 | 17.3 | 21.1 | 1.1 | 0.8 | 2.4 | 2.1 | 6.4 | 7.8 | 2.70 | ||
a
DNA samples and fragments written from 5′ to 3′ with p indicating the position of terminal phosphate groups.
b
Total damage includes all losses of initial targeted molecules based on HPLC-UV analysis. The values are expressed as a ratio of damaged molecules to 1000 initial target molecules and are the average of three independent experiments; SD= ± 20%.
c
Percentage of C-Br cleavage based on total damage.
d
Percentage of C-N cleavage based on total damage.
e
Percentage of C-O cleavage based on total damage.
f
Ratio of C-N to C-O cleavage.