Figure 1.

Expression of candidate tumor-suppressor genes that negatively regulate RAS signaling in thyroid cancer cell lines. A) Reverse-transcription analysis of a cDNA panel derived from 12 human thyroid cancer cell lines as indicated. The cDNA from normal human thyroid tissue and two immortalized normal human thyroid epithelial cell lines (Htori-3 and TAD2) were used as positive controls. ß-Actin cDNA was used to show the integrity and quantity of cDNA samples. The primer sequences are presented in Supplementary Table 2 (available online). B) Detection of RASAL1 protein in thyroid cell lines by western blotting assay. ß-Actin protein level was used to show the quantity and integrity of protein samples.