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Endothelial TNFR1 expression in human brain metastasis. Large insets are magnified images of boxed areas. A) Positive control section showing tumor necrosis factor receptor 1 (TNFR1) expression (brown) on a brain vessel adjacent to nonspecific inflammation. B) Normal brain tissue showing minimal TNFR1 reaction in cortical vessel. C and D) Staining for TNFR1 on brain parenchymal endothelial cells at sites of metastasis. Scale bars are 100 μm (A–D) or 20 μm (insets).
