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. 2013 Nov 5;11(11):e1001704. doi: 10.1371/journal.pbio.1001704

Figure 7. WASP and N-WASP negatively regulates each other.

Figure 7

(A–D) TIRFM analysis of pWASP and pN-WASP in the contact zone of splenic B cells stimulated with membrane-tethered Fab′–anti-Ig (A and B). The MFI of pWASP (C) or pN-WASP (D) in the B-cell contact zone was quantified. (E–G) Flow cytometry analysis of the cellular MFI of pWASP or pN-WASP in splenic B cells from WKO and littermate control mice (F), and mouse splenic (E) and human PBMC B cells (G) that were treated with or without Wis and soluble mB-Fab′–anti-Ig plus streptavidin. (H) Flow cytometry analysis of the cellular MFI of pN-WASP in PBMC B cells from WAS patients and age-matched healthy donors that were incubated with or without soluble mB-Fab′–anti-Ig plus streptavidin for 2 min. Shown are representative images at 7 min and the average MFI (±SD) from three independent experiments. Bars, 2.5 µm. * p<0.01, compared to B cells from wt or littermate control mice, without Wis treatment or healthy donors.