Figure 2. The bpss1439 mutant displayed reduced biofilm formation which was complemented by in trans bpss1439 expression.

a. The wild-type and bpss1439 mutant were grown in LB under static conditions at 37°C for 48 hours. Biofilms were stained with 1% w/v crystal violet, solubilised and quantified using an optimal density reading at 595 nm. Results are plotted with standard deviation error bars from triplicate experiments each consisting of eight experimental replicates; the p value was calculated using a paired Student's T test. b. The trans-complemented bpss1439 mutant (pME-1439), as well as the wild-type and bpss1439 pME strain, were also assessed for biofilm production as described previously.