|
B. pseudomallei: |
|
|
| 10276 |
Clinical isolate (wild-type) |
Dr T. Pitt (Public Health Laboratory Service, London, UK) |
| 10276 (pME) |
10276 harbouring the pME6032 plasmid |
This study |
|
bpss1439 (bbfA) mutant |
10276 harbouring a pKNOCK-Kan single cross-over insertion in bpss1439 (bbfA)
|
This study |
|
bpss1439 (bbfA) mutant (pME) |
10276 harbouring a pKNOCK-Kan single cross-over insertion in bpss1439 (bbfA) and the pME6032 plasmid |
This study |
|
bpss1439 (bbfA) mutant (pME-1439) |
10276 harbouring a pKNOCK-Kan single cross-over insertion in bpss1439 (bbfA) and the pME6032 plasmid containing a full-length copy of bpss1439
|
This study |
|
E. coli: |
|
|
| S17-1 λpir
|
Laboratory strain (λpir hsdR pro thi; chromosomal integrated RP4-2 Tc::Mu Km::Tn7). |
[36]
|
| Plasmids: |
|
|
| pKNOCK-Kan |
2.2 kb, KanR, λpir-dependent vector (oriR6K) with RP4 oriT
|
[23]
|
| pKNOCK-Kan-bpss1439
|
pKNOCK-Kan containing a 623 bp internal fragment of bpss1439 (bbfA)
|
This study |
| pME (pME6032) |
9.8 kb, TetR, pVS1 derived shuttle vector with IPTG inducible ptac promoter |
[24]
|
| pME-1439
|
pME6032 containing the full-length (4593 bp) bpss1439 (bbfA) gene, including 146 bp upstream |
This study |
|
Eukaryotic cell lines:
|
|
|
| J774.2 |
Murine macrophage-like cells |
ECACC 85011428 |
| A549 |
Human lung airway epithelial cells |
ECACC 86012804 |
