Table 1. Summary of the routine confocal checks performed with different objective lenses.
Performance test
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Objective lens | Laser power | Laser stability | Axial resolution and colocalisation | Field illumination | Bead colocalisation | Point spread function (PSF)nbsp; | Lambda scan(1) | XY galvos | XY stage | Z galvo stability(1) |
10× | ● | ● | ● | ● | ● | ● | ● | ● | ||
20× | ● | ● | ● | ● | ● | |||||
40× | ● | ● | ● | ● | ●* | |||||
63× | ● | ● | ● | ● | ●* | |||||
100× | ● | ● | ● | ● | ●* |
* Z galvo stability is only tested with one of the high NA objectives.
(1) These measurements are only applicable to the Leica SP confocal systems SP1/SP2/SP5.