Figure 1. Atf1 and Pcr1 are required for efficient maltose utilization.

(A) Fission yeast spores fail to form colonies when grown on maltose-containing medium. Wild-type (mating-type: h ⁹⁰) tetrads were dissected on YE (glucose-containing) and YEM (maltose-containing) plates, and incubated at 30°C for 3 days. (B) atf1∆ and pcr1∆ mutants grow slowly on maltose-containing plates. Wild-type, atf1∆, and pcr1∆ strains were streaked on plates containing the indicated sugar(s) and incubated at 30°C for 3 days. (C) Growth of atf1∆ and pcr1∆ mutants does not recover following switching from glucose- to maltose-containing medium. Cells of the indicated strains exponentially growing in YE were washed twice, resuspended in YE or YEM, and incubated at 30°C for the indicated number of hours.