Figure 5. Med7 associates with its target genes upon switching of the carbon source from glucose to maltose in an Atf1- and Pcr1-dependent manner.

(A) Med7 associates with agl1 in maltose-utilizing cells. Cells expressing the indicated proteins were cultured as described in Figure 3B and used for ChIP analysis. The levels of the indicated proteins at the target regions of agl1 (presented in Figure 3A) were normalized against the levels at act1. Glc: glucose; Mal: maltose. *P<0.05, **P<0.01 (Student's t-test). (B) The increased Med7 occupancy at agl1 depends on Atf1 and Pcr1. Cells of the indicated strains were cultured as described in Figure 3B and used for ChIP analysis. The levels of Med7 associated with the indicated genes relative to the level of Med7 associated with act1 in cells grown in maltose-containing medium. The fold change compared to levels in cells grown in glucose-containing medium is shown. Error bars, s.d. (n=3).