Table 1. Antibodies used for Western blotting.
Antigen | Host | P/Ma | Dilution | Supplier | Catalog no. |
---|---|---|---|---|---|
pPDK1 (Ser-241) | rabbit | P | 1: 1,000 | Cell Signaling | 3061 |
Synapsin I | rabbit | P | 1:10,000 | Millipore | AB1543P |
pSynapsin I (Ser-9) | rabbit | P | 1: 1,000 | Cell Signaling | 2311S |
pSynapsin I (Ser-603) | rabbit | P | 1: 8,000 | Sigma-Aldrich | S8192 |
pDARPP-32 (Thr-34) | rabbit | M | 1: 5,000 | Cell Signaling | 5393S |
CRMP2 | rabbit | P | 1: 1,000 | Millipore | AB9218 |
pCRMP2( Thr-514) | rabbit | P | 1: 1,000 | Cell Signaling | 9397 |
pCRMP2 (Ser-522) | rabbit | P | 1: 1,000 | ECM Biosciences LLC | CP2191 |
Dynamin-1 | mouse | M | 1:8,000 | Cell signaling | 4565 |
pDynamin-1 (Ser-778) | sheep | P | 1: 2,000 | Thermo scientific | PA1-4621 |
pDynamin-1 (Ser-795) | rabbit | P | 1: 2,000 | Santa Cruz Biotechnology | SC-12937 |
Tubulin | rabbit | P | 1:16,000 | Cell Signaling | 2148 |
Ac-Tubulin (Lys-40) | rabbit | P | 1: 1,000 | Cell Signaling | 3971S |
Phosphotyrosine antibody (clone 4G10) | mouse | M | 1: 4,000 | Millipore | 05-321 |
PIMT | rabbit | P | 1:2,500 | Custom | |
β-actin | rabbit | M | 1:30,000 | Cell Signaling | 4970S |
a Polyclonal or Monoclonal