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. 2013 Nov 5;8(11):e80752. doi: 10.1371/journal.pone.0080752

Figure 4. Rrp6 can be overexpressed in wild-type and rrp47∆ strains.

Figure 4

Western analyses of Rrp6 in isogenic wild-type and rrp47∆ strains that are transformed with RRP6 expression constructs. Strains were transformed with either the 2 micron (2μ) vector pRS426 (lane 1), the centromeric (cen) plasmid pRS416 (lane 2), an RRP6 genomic clone in pRS416 (lane 3) or in pRS426 (lane 4), as well as constructs expressing an N-terminal zz fusion of Rrp6 from the RRP4 promoter in pRS416 (lane 5) or pRS426 (lane 6). Identical blots were analysed for Rrp6 levels using an Rrp6-specific antiserum, followed by analysis of the loading control Pgk1.