Figure 6. Exogenous expression of Rrp6 complements the synthetic lethality of rrp47∆ rex1∆ and rrp47∆ mpp6∆ mutants.

Yeast rrp47∆ rex1∆ and rrp47∆ mpp6∆ double mutants bearing plasmids with a URA3 marker and a wild-type copy of either the RRP47 (panels A-C) or MPP6 gene (panel D) were transformed with RRP6 constructs. Transformants were isolated on selective minimal medium and tested for growth in parallel on permissive minimal medium (left panel) and on medium containing 5 FOA (right panel). Plates were incubated at 30 °C for 3 days. The nature of the expression construct is indicated for each segment on the right.