Figure 8. Northern analyses of rrp47∆ rex1∆ and rrp47∆ mpp6∆ mutants.

Total cellular RNA was isolated from isogenic wild-type, rex1∆, rrp47∆ and mpp6∆ strains, and from rrp47∆ rex1∆ rrp47∆ or rrp47∆ mpp6∆ double mutants that are complemented by centromeric (cen) or 2 micron (2μ) plasmids expressing RRP47, MPP6 or RRP6 alleles. RRP6 constructs encoded either non-tagged or zz-tagged fusion proteins. RNA was resolved through 8% denaturing polyacrylamide gels and northern blot analyses performed, using probes complementary to the RNAs indicated on the right of each panel. (A-F) Analysis of rrp47∆ rex1∆ mutants. (G-N) Analysis of rrp47∆ mpp6∆ mutants. To compare the relative levels of both mature and 3’ extended forms of snoRNAs in the different strains in panels A-C, two images are shown from the same hybridisation. Dispersed bands labelled I-pA and II-pA represent snoRNAs that are polyadenylated after termination at sites I or II, respectively. Bands labelled 5S* and snR13* represent truncated RNAs.