Table 2.
Sequence of oligonucleotide primers for quantitative RT-PCR analysis
| Gene name | Size (bp) | Accession No. | Primer sequence |
|---|---|---|---|
| ACC | 192 | NT_033778 | F:5'-GGAAAACGGTCAGGTACGGA-3' |
| R:5'-TTCGGACGTTGTATGCACCA-3' | |||
| G6PDH | 128 | NC_013047 | F:5'-TCACCTTGTGCTGTCCGTTT-3' |
| R:5'-CGATCGGGCTCACACAAGTA-3' | |||
| L-PK | 233 | NC_005101 | F:5'-CCACCAGGCACAGAAACACT-3' |
| R:5'-TGAGCTTGGCAGAGGATGAC-3' | |||
| GK | 144 | NM_001124249 | F:5'-CCAGTGTCAAAATGCTGCCC-3' |
| R:5'-CCTTCCATCCCCTCTCCTCA-3' | |||
| SCD-1 | 373 | NM_139192 | F:5'-CTAGGTGGGTGGCTGTTGAG-3' |
| R:5'-ATGTAACTGCGGGAAGGGTG-3' | |||
| D5D | 108 | NC_005100 | F:5'-CCACTACGCTGGTCAGGATG-3' |
| R:5'-GCGCTTGCATTCTGGGTCTA-3' | |||
| FAS | 168 | NC_005100 | F:5'-CTAGGCGGGGAACTGCTTTC-3' |
| R:5'-TCTGGATCTGTGGAGTGGCT-3' | |||
| ChREBP | 420 | NC_005111 | F:5'-GGCGAAGGGAATTCAGGACA-3' |
| R:5'-GGGCCACCCGTTTAGCTATC-3' | |||
| PPARα | 164 | NC_005106.3 | F:5'-CAAACCATCCTAGTGTGCCCT-3' |
| R:5'-CCACAGAGGGCAGCATTTCT-3' | |||
| PGC1α | 102 | NC_005113.3 | F:5'-ACTCTTGAGGGCTTACAACCT-3' |
| R:5'-AGCACTGGGCTTATTTGTGGA-3' | |||
| CPT1 | 308 | NC_005106 | F:5'-CCTCACCACTCCAATCCCAC-3' |
| R:5'-CGTCCCGAAATCGAGTCTGA-3' | |||
| ACS | 173 | NC_003076 | F:5'-GCCCCAATCTGGAAATGGCA-3' |
| R:5'-CTTACAGCGAGGAGCTTCGT-3' | |||
| FGF21 | 119 | NC_005100.3 | F:5'-TGCAGGCCTCAGGATCAAAG-3' |
| R:5'-GGCTGGACCTGAATCTGGAG-3' | |||
| ACOX | 246 | NC_002754 | F:5'-GGGCAGTATACAAAGGGGCA-3' |
| R:5'-CCCCTACATACCAACCGCTC-3' | |||
| HSL | 250 | NC_005100 | F:5'-GGGCTGCTAAGCTCAGGAAA-3' |
| R:5'-TCGGTTAACCACAGCTTCCC-3' | |||
| β-Actin | 108 | NC_005111 | F:5'-AGAAGGGACACCGTAGAGGG-3' |
| R:5'-GCGGGAATACGACTGCAAAC-3' |
F, forward; R, reverse; ACC, acetyl CoA carboxylase; G6PDH, glucose-6-phosphate dehydrogenase; LPK, liver pyruvate kinase; GK, glucokinase; SCD1, stearoyl-CoA desaturase 1; D5D, Δ5 desaturase; FAS, tumor necrosis factor receptor superfamily member 6 (TNFRSF6); ChREBP, carbohydrate responsive element binding protein; PPARα, peroxisome proliferator activated receptor alpha; PGC1α, peroxisome proliferator activated receptor gamma co-activator alpha; CPT1, carnitine palmitoyltransferase 1; ACS, acetyl-CoA synhtetate; FGF21, fibroblast growth factor 21; ACOX, acyl-CoA oxidase; HSL, hormone sensitive lipase; β-actin.