Figure 1. Immunogenicity of T790M-derived peptides in PBMCs from HLA-A2⁺ healthy donors.

The immunogenicity of T790M-deived peptides, T790M-5, T790M-7, and T790M-8, was examined with PBMCs from 6 different HLA-A2⁺ healthy donors. PBMCs were stimulated 5 times with T790M-5, T790M-7, or T790M-8 peptides (10 µg/ml) every 3 or 4 days. The stimulated PBMCs (2×10⁴ cells/well) were examined for reactivity against T2 cells (1×10⁴ cells/well) pulsed with the corresponding peptides or control HIV peptide (10 µg/ml) by IFN-γ ELISPOT assay. The assays were carried out in duplicate wells, and representative wells in each donor are shown. The numbers of spots are shown for each well. Positive antigen-specific T cell responses are marked by closed boxes.