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. 2013 Nov 5;8(11):e78389. doi: 10.1371/journal.pone.0078389

Figure 3. Cytotoxicity of the T790M-5- or T790M-7-stimulated T cells against NSCLC cells harboring EGFR T790M mutation.

Figure 3

(A) The T790M-5- or T790M-7-stimulated PBMCs were examined for their cytotoxicity against the NSCLC cell lines (2×103 cells/well), NCI-H1975 (HLA-A2− T790M+) or NCI-H1975-A2 (HLA-A2+ T790M+), at the indicated effector/target ratios. (B) The T790M-5- or T790M-7-stimulated PBMCs (1×104 cells/well) were examined for their cytotoxicity against the NSCLC cell lines (2×103 cells/well), PC9 (HLA-A2+ T790M−), PC9/ZD (HLA-A2+ T790M+) or HCC827 (HLA-A2− T790M−), at an effector/target ratio of 5∶1. The assays were carried out in duplicate, and the means of duplicate samples were used for calculation. The experiments were repeated with blood from three different donors, and a representative result is shown.