Table 1. HLA-A2-binding capability of the predicted EGFR T790M-derived peptides.

Peptide name	Amino acid sequence	NetMHC 3.2 ANN IC50 (nM)	BIMAS score	HLA-binding capability (%)a
T790M-1	VQLIMQLMPF	3705	0.109	0
T790M-2	QLIMQLMPFG	4054	0.943	0
T790M-3	LIMQLMPFGC	837	24.921	0
T790M-4	IMQLMPFGCL	925	6.478	0
T790M-5	MQLMPFGCLL	977	51.770	207.5
T790M-6	LTSTVQLIMQL	4187	NAc	0
T790M-7	LIMQLMPFGCL	530	NA	19.1
T790M-8	IMQLMPFGCLL	118	NA	57.0
WT-5b	TQLMPFGCLL	2578	30.453	158.2
WT-7b	LITQLMPFGCL	5063	NA	0
Flu-M1	GILGFVFTL	18	550.927	234.4

^aHLA-A2-binding capability was estimated by the increase in mean fluorescence intensity (MFI) as determined by flow cytometry after staining of RMA-S/A2 cells with anti-HLA-A2 mAb, as follows: MFI increase (%)  =  (MFI with a given peptide – MFI without peptides)/(MFI without peptides) X 100. The experiments were repeated three times and a representative result is shown.

^bWT-5 and WT-7 peptides have wild-type sequences, corresponding to the T790M-5 and T790M-7 peptides, respectively.

^cNA, not available.