Figure 7. The motor fuction changes between control and PERK-siRNA groups in each time point under ¹²⁵I radiation.

(A) Porcines transfected with RNAi for PERK and controls were radiated with ¹²⁵I for 6 months. Protein levels were measured by immunoblot analysis using antibodies against LC3II and β-actin at each time point of 1 week, 1 month, 3 months and 6 months. (B) Annexin V-FITC/PI double staining analysis at different times. The lower left quadrant (low-fluorescence PI and FITC signals) contains normal viable cells and the lower right quadrant (low-fluorescence PI and high-fluorescence FITC signals) defines cells in the early stages of apoptosis. The two upper quadrants (high-fluorescence PI signal) define dead cells. The numbers indicate the percentages of the different populations of cells.