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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Int Immunopharmacol. 2013 Aug 28;17(3):10.1016/j.intimp.2013.08.005. doi: 10.1016/j.intimp.2013.08.005

Figure 4.

Figure 4

Subsets of lung GR1+ cells express Sema4A and Tim-2. Single cell suspensions obtained from lungs of Alum- or OVA-treated mice were analyzed for GR1, Sema4A, and Tim-2 expression using corresponding Abs defined in Materials and Methods section. (A) Approximately a half of lung GR1+ cells co-expressed Sema4A. (B) Tim-2+ cells were selected for further analysis based on SSC-Fl3 dot plots (not shown) and then re-gated on Fl1-Fl3 density plots. Note that GR1+Tim2+ cells appear only under inflammatory lung conditions.