Table 2.
Antibodies used for immunohistochemistry
| Antibody | Pretreatment | Incubation | Detection | Dilution | Company | |
|---|---|---|---|---|---|---|
| AMACR |
Monoclonal |
98°C Hot water, pH 9.0, |
15 min |
Envision™ |
Ready-to-Use Kit |
Dako, Glostrup, Denmark |
| p16Ink |
Monoclonal, clone G175-405 |
98°C Hot water, pH 6.1, |
20 min |
Envision™ |
1:10 |
BD Biosciences, Heidelberg, Germany |
| Ki67 |
Monclonal, clone MIB-1 |
98°C Hot water, pH 9.0, |
15 min |
Envision™ |
Ready-to-Use Kit |
Dako, Glostrup, Denmark |
| BRAF |
Monoclonal, clone VE1 |
37°C Hot water, PH 9.0 |
30 min |
Envision™ |
1:100 |
DCS Innovative Diagnostik, Hamburg, Germany |
| MLH1 |
Monoclonal, clone ES05 |
98°C, pH 9.0 |
20 min |
Envision™ |
Ready-to-Use Kit |
Dako, Glostrup, Denmark |
| Beta-catenin |
Monoclonal |
37°C water bath 30 min |
20 min |
Zytochem Plus HRP Polymer (Zytomed Systems, Berlin, Germany) |
1:2000 |
BD Biosciences, Heidelberg, Germany |
| CK20 | Monoclonal, Clone Ks20.8 | 98°C, pH 9.0 | 30 min | Envision™ | Ready-to-Use Kit | Dako, Glostrup, Denmark |