Figure 4. Relative proportions of SfNIC-Begt or SfNIC-Bp10 and SfNIC-C viruses in OBs comprising A) SfNIC-Begt:SfNIC-C and B) SfNIC-Bp10:SfNIC-C co-occluded mixtures at proportions of 90∶10, 75∶25, 50∶50, 25∶75 and 10∶90.

The relative proportion of each virus was determined by qPCR using DNA extracted from co-occluded mixed genotype OBs obtained after injection of S. frugiperda fourth instars using ODVs released from OB mixtures at the desired proportions. Primers qSfBpif1.F and qSfBpif1.R were used for amplification of SfNIC-Begt and SfNIC-Bp10, that did not amplify in SfNIC-C. Primers qSfCcath1.F and qSfCsf361.R were used for amplification of SfNIC-C.