. Author manuscript; available in PMC: 2013 Nov 6.

Published in final edited form as: J Immunol Methods. 2009 Mar 10;344(1):10.1016/j.jim.2009.02.007. doi: 10.1016/j.jim.2009.02.007

Table 1.

Characteristics of the automated ELISpot compared with the manual process.

	Automated ELISpot assay	Manual ELISpot assay
Throughput	High	Limited
Precision/reproducibility	Mean CV=27.2%, SD=22.3%^*	Mean CV=36.2%, SD=35.2%^*
	No plate location effects	More subject to high CV at low SFUs
Sample handling	Minimal	Labour intensive
Plate design and documentation	Automatically recorded and trackable	Operator dependent

CVs measured in this study using anti-CEF responses in healthy subjects.